Application

Immunohistochemistry Analysis: A 1:50-2,000 dilution from a representative lot detected Mastl in human prostate cancer and human testis tissue sections.

Detect Mastl using this mouse monoclonal antibody, Anti-Mastl Antibody, clone 4F9 validated for use in western blotting & IHC.

Research CategoryCell Structure

Research Sub CategoryAdhesion (CAMs)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Mastl is also called Serine/threonine-protein kinase greatwall (GW) and Microtubule-associated serine/threonine-protein kinase-like (MAST-L). Mastl is a serine/threonine kinase that regulates mitosis entry and maintenance during M phase through the inactivation of protein phosphatase 2A (PP2A). Mastl is also thought to be involved in megakaryocyte differentiation and the diseaseThrombocytopenia 2 (THC2).

Immunogen

Epitope: C-terminus

Recombinant protein corresponding to the C-terminus of human Mastl.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in human oral squamos cell carcinoma lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Mastl in 10 µg of human oral squamos cell carcinoma lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~97 kDa observed. Uncharacterized band(s) may appear in some lysates.